Evaluation of equine aqueous humor protein

ABSTRACT Evaluation of equine aqueous humor (AH) proteins can help the diagnosis of uveitis. However, studies on proteins in this fluid in healthy horses are scarce and present variations according to the methodology employed. This study aimed to perform protein analysis and cytology of equine aqueous humor of healthy horses and verify its correlation with plasmatic proteins. Thirteen adult horses (26 eyes), mixed breed, male or female were evaluated. A volume of 0.5 mL of aqueous humor was collected through aqueocentesis from both eyes. The samples were submitted to protein quantification by modified Bradford method and to sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE), and to cytological evaluation. Blood was collected for determination of plasmatic protein concentration. Thirteen eyes (50% of the samples) had values larger than zero by the Bradford method, with an average of 40.3 mg/dl±6.45. Electrophoresis showed presence of higher masses of proteins (43 KDa). There were no cells in 96.15% of the samples (25 eyes). Healthy equines presented low protein concentration in the HA. The ratio between protein concentration in the aqueous humor / total plasma protein of 0.56%.

**摘要** 马房水（aqueous humor, AH）蛋白质检测有助于葡萄膜炎的临床诊断。然而，针对健康马房水蛋白质的相关研究较为匮乏，且现有研究结果因采用的实验方法不同而存在差异。本研究旨在对健康马的房水开展蛋白质分析与细胞学检测，并探究其与血浆蛋白的相关性。本研究共纳入13匹成年混血马（共26只眼），雌雄不限。通过双眼房水穿刺术采集0.5 mL房水样本，分别采用改良Bradford法进行蛋白定量、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析以及细胞学检测；同时采集血液样本以测定血浆蛋白浓度。检测结果显示，13只眼（占总样本的50%）的Bradford法蛋白定量值大于0，平均浓度为40.3 mg/dl±6.45。电泳分析可见分子量为43 KDa的高丰度蛋白。96.15%的样本（25只眼）未检测到细胞成分。健康马房水的蛋白浓度较低，房水蛋白浓度与血浆总蛋白浓度的比值为0.56%。