Cryo-electron microscopy EPN BAG

At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turning to cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. In a nutshell, there are more than 45 PIs that could benefit from Krios time on the EPN campus (more than 90 different samples). Around 20 % of the sample are ready for high resolution data collection with already prepared grids that have been pre-screened on Glacios microscopes. Whereas some work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level

在EPN园区内，IBS、ESRF、ILL及EMBL这4家研究所均开展结构生物学研究工作。当前，所有结构生物学研究团队均已转向冷冻电子显微镜（cryo-EM）技术，因此园区内对该类设备的需求极为旺盛，且覆盖全部4家研究所。简言之，EPN园区内共有超过45名首席研究员（Principal Investigator，PI）可从Krios系列冷冻电镜机时中获益，相关研究涉及90余种不同样品。其中约20%的样品已完成制备，可直接开展高分辨率数据采集，其制备好的载网已在Glacios显微镜上完成预筛查。尽管部分实验可在Glacios显微镜上完成，但对于异质样品、浓度受限需大量成像的样品或小型蛋白等疑难样本，使用Krios显微镜则不可或缺。仅此类样品将被纳入光束线分配组（BAG）层面的Krios机时申请遴选范围。