Raw data for the manuscript under the title "Mitochondrial RNA granules are fluid condensates, positioned by membrane dynamics".

<strong>This is the data-repository</strong> to contain all relevant raw-data used and referred to in the manuscript entitled:<br> "Mitochondrial RNA granules are fluid condensates, positioned by membrane dynamics"<br> [manuscript under revision, and thus not citable as published article] The repository is structured analogous to the manuscript and holds the data for: <strong>Figure 1</strong>: 1-colour STORM data, 2-colour STORM data<br> What should be considered the raw-data for SMLM-studies is a matter of debate. While the localisation-software as well as the specified parameters used therein can greatly influence the final analysis, in principle all raw-images should be provided as raw-data. However, this would result in excessive data-volumes required for our study. We therefore decided to upload only raw-localisations here. Raw image-stacks can be provided upon reasonable request to the authors directly.<br> For all<strong> 1C-STORM</strong> acquisitions, Alexa-647 was used and localisations were extracted from raw images using sCMOS adapted software [Huang F. et al., Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms <em>Nat Methods</em> 7, 654:658, doi:10.1038/nmeth.2488 (2013)] as previously described here: Douglass, K. M. et al. Super-resolution imaging of multiple cells by optimised flat-field epi-illumination. <em>Nat Photonics 10</em>, 705–708, doi:10.1038/nphoton.2016.200 (2016), and here: Sieben, C. et al. Multicolor single-particle reconstruction of protein complexes.<em> Nat Methods</em> 15, 777–780, doi:10.1038/s41592-018-0140-x (2018). We used <strong>kernel-sizes = 3, 7, and 3</strong> for all data &amp; the <strong>threshold 40</strong> for BrU-data and<strong> threshold = 100</strong> for all other data-sets.<br> In<strong> 2C-STORM,</strong> BrU-localisations were generated analogous to 1C, while FASTKD2 was labelled with dyLight 755 secondary antibodies. Here, the<strong> kernel-sizes </strong>were set to<strong> 3, 7, 3 and thresold = 27</strong> for data acquired in 2018, whereas the more recent data was found to be less bright at the same microscope-settings and thus the localisation parameters for late 2019 &amp; early 2020 data were set to <strong>3, 5, 3 for kernel</strong> sizes and <strong>10 as a threshold</strong>-value. Figure 2: FRAP- and live-cell SIM-data Figure 3 &amp; 4: EM-, STED-, and SIM-data