Data from: Symbiont infection and psyllid haplotype influence phenotypic plasticity during host switching events

# Data from: Symbiont infection and psyllid haplotype influence phenotypic plasticity during host switching events [https://doi.org/10.5061/dryad.xksn02vpp](https://doi.org/10.5061/dryad.xksn02vpp) This dataset includes raw electropenetrography (EPG) recordings used to disentangle the effects of host acclimation, Candidatus Liberibacter solanacearum (CLso) infection, and psyllid haplotype on the within-plant feeding behavior of Bactericera cockerelli during conspecific and heterospecific host switches. This approach allows to connect phenotypic plasticity with the role of B. cockerelli as a vector by quantifying the frequency and duration of behaviors involved in CLso transmission. ## Description of the data and file structure The EPG recordings have the following naming scheme: Folder "Central_Lso+_mt_yp" refers to EPG recordings of psyllids of the Central haplotype_infected by CLso_reared on MicroTom tomato_trasferred to Yellow Pear tomato. The same naming scheme applies to individual recordings (subfolders), which are uniquely identified by a recording number at the end of the file name. Inside each subfolder there are 14 hourly recordings labelled as ".D01" to ".D14" The EPG recordings are structured as follows: ├── Psyllid haplotype_CLso infection status_rearing host_transfer host (Main folder) ├── Psyllid haplotype_CLso infection status_rearing host_transfer host_recording number (Subfolder) ├── Psyllid haplotype_CLso infection status_rearing host_transfer host_recording number_hour number (hourly recordings) For example: ├── Central_Lso+_mt_yp ├── Central_Lso+_mt_yp_01 ├── Central_Lso+_mt_yp_01.D01 The EPG recordings have been used for three experimental comparisons: | **Experimental comparison** | **Psyllid haplotype** | **Infection status** | **Rearing host** | **Novel host** | **Novel host** | | :-------------------------- | :-------------------- | :------------------- | :--------------- | :------------- | :------------- | | 1 | Western | uninfected | Bell Pepper | Yellow Pear | MicroTom | | | Western | uninfected | MicroTom | Yellow Pear | Bell Pepper | | 2 | Western | CLso+ | MicroTom | Yellow Pear | Bell Pepper | | | Western | uninfected | MicroTom | Yellow Pear | Bell Pepper | | 3 | Western | CLso+ | MicroTom | Yellow Pear | Bell Pepper | | | Central | CLso+ | MicroTom | Yellow Pear | Bell Pepper | ## Code/Software The EPG recordings (hourly files) can be opened by using the "Stylet+ a" Windows software (EPG systems) to mark the beginning of each of the following waveforms: NP: non-probing behaviour; C: stylet pathways in mesophyll and parenchyma; D: initial contact with phloem cells, marked as ‘11’; E1: phloem salivation; E2: passive phloem sap ingestion; G: active xylem ingestion. Files with waveform sequences were then uploaded to the Microsoft Excel Workbook for automatic parameter calculation of EPG data originally developed for aphid waveforms by Sarria et al. (2009) to obtain the following variables: number, duration per insect and duration per event for NP, C, D (as pd-L in the worksheet), E1, E2 and G waveforms; number of single E1 (i.e., E1 followed by C instead of E2); number of sustained E1 (sE1) and E2 (sE2), that is, E1 and E2 events longer than 10 min, respectively; percentage of probing time spent in C, G, E1 and E2 phases and percentage of sE2 over total E2. A separate workbook was used for each insect/plant treatment, and the variables thus cal- culated by individual insects were averaged by treatment using the Summarize with PivotTable function of Excel.

# 数据来源：共生体感染与木虱单倍型对寄主转换过程中表型可塑性的影响 [https://doi.org/10.5061/dryad.xksn02vpp]
本数据集包含原始电刺探电位图谱（electropenetrography, EPG）记录，用于解析寄主驯化、韧皮部杆菌属未定种（Candidatus Liberibacter solanacearum, CLso）感染以及木虱单倍型对番茄木虱（Bactericera cockerelli）在同种及异种寄主转换过程中植株内取食行为的影响。本研究通过量化CLso传播相关行为的发生频率与持续时长，将表型可塑性与番茄木虱作为传毒媒介的功能建立关联。

## 数据与文件结构说明
EPG记录采用如下命名规则：主文件夹"Central_Lso+_mt_yp"对应感染CLso、在MicroTom番茄上饲养后转移至Yellow Pear番茄的Central单倍型木虱的EPG记录。该命名规则同样适用于单个记录子文件夹，子文件夹名称末尾以记录编号作为唯一标识。每个子文件夹内包含14份每小时记录文件，命名为".D01"至".D14"。

EPG记录的结构如下：
├── 木虱单倍型_CLso感染状态_饲养寄主_转接寄主（主文件夹）
├── 木虱单倍型_CLso感染状态_饲养寄主_转接寄主_记录编号（子文件夹）
├── 木虱单倍型_CLso感染状态_饲养寄主_转接寄主_记录编号_小时编号（每小时记录文件）

例如：
├── Central_Lso+_mt_yp
├── Central_Lso+_mt_yp_01
├── Central_Lso+_mt_yp_01.D01

本数据集的EPG记录用于三项实验比较，具体如下：
| **实验比较组** | **木虱单倍型** | **感染状态** | **饲养寄主** | **新寄主1** | **新寄主2** |
| :-------------- | :------------- | :----------- | :----------- | :---------- | :---------- |
| 1 | 西方单倍型 | 未感染 | 甜椒 | Yellow Pear番茄 | MicroTom番茄 |
| | 西方单倍型 | 未感染 | MicroTom番茄 | Yellow Pear番茄 | 甜椒 |
| 2 | 西方单倍型 | CLso感染阳性 | MicroTom番茄 | Yellow Pear番茄 | 甜椒 |
| | 西方单倍型 | 未感染 | MicroTom番茄 | Yellow Pear番茄 | 甜椒 |
| 3 | 西方单倍型 | CLso感染阳性 | MicroTom番茄 | Yellow Pear番茄 | 甜椒 |
| | 中央单倍型 | CLso感染阳性 | MicroTom番茄 | Yellow Pear番茄 | 甜椒 |

## 代码与软件工具
本数据集的每小时EPG记录文件可通过Windows平台的"Stylet+ a"（EPG系统配套软件）打开，用于标记以下各类波形的起始时刻：
- NP：非刺探行为（non-probing behaviour）
- C：叶肉与薄壁组织内的口针通路
- D：与韧皮部细胞的初始接触，标记为"11"
- E1：韧皮部唾液分泌
- E2：被动吸收韧皮部汁液
- G：主动吸收木质部汁液

包含波形序列的文件将上传至Microsoft Excel工作簿，通过Sarria等人（2009）为蚜虫EPG数据开发的自动参数计算工具，获取如下变量：
针对NP、C、D（工作表中记为pd-L）、E1、E2及G波形的事件总数、单虫取食时长、单次事件时长；
单次E1事件数量（即E1事件后接C而非E2的情况）；
持续型E1（sE1）与持续型E2（sE2）的数量，分别指时长超过10分钟的E1与E2事件；
取食总时长中用于C、G、E1及E2阶段的占比，以及sE2占总E2时长的比例。

每一组昆虫-寄主处理对应一个独立的Excel工作簿，随后通过Excel的"数据透视表汇总"功能，将单虫计算得到的变量按处理组进行平均。