Data underlying the manuscript: Self-mixing in microtubule-kinesin active fluid from nonuniform to uniform distribution of activity

This database presents the data used in the manuscript: Self-mixing in microtubule-kinesin active fluid from nonuniform to uniform distribution of activity (Bate et al. <em>Nat Commun</em> <strong>13</strong>, 6573 [2022]). This database contains two sets of data: (1) images of tracer particles suspended in active-inactive fluid systems and (2) images of fluorescein suspended in active-inactive fluid systems. Each data set is provided with associated analysis script for demonstrating the process of extracting mixing-related parameters from the data, such as interface progression exponents, interface progression coefficients and mixing times. <strong>The first data set</strong> contains 4 files: Data1.zip, analysis1.m, Mask.tif, and Tracking.mat. Data1.zip contains 1 hour of frame-by-frame tracer data from an individual experiment in the [cATP] sweep in Fig. 2c of the manuscript, for the sample with 5 mM caged ATP. The tracers are tracked using the tracking software developed by Ouellette et al. (Experiments in Fluids <strong>40</strong>, 301-313 [2005]) and the tracking results are output in Tracking.mat, which can be subsequently imported to MATLAB by running analysis1.m (requiring MATLAB Curve Fitting Toolbox, Image Processing Toolbox and Parallel Computing Toolbox). The script outputs the interface progression exponents and interface progression coefficient of this data set. The script also allows for analyzing a specific region of interest defined as the black pixels in Mask.tif. In this analysis, the region of interest is set to be the entire image. <strong>The second data set</strong> contains 2 files: Data2.zip and analysis2.m. Data2.zip contains 1 hour of frame-by-frame fluorescein data from an individual experiment in the fluorescein sweep in Fig. 5 of the manuscript. The sample contains 3.2 mM caged ATP, 3 µM caged fluorescein, 80 nM K365 dimers and the sample height is 100 µm. This experiment is designed to probe the mixing time of suspended fluorescein in the active fluid system. To extract the mixing time, Data2.zip needs to be unzipped and analysis2.m needs to be run in MATLAB (requiring MATLAB Curve Fitting Toolbox and Image Processing Toolbox), which will read the fluorescein images to analyze the multiscale norm and output the mixing time.

本数据库收录了论文《微管-驱动蛋白活性流体中从活性非均匀分布到均匀分布的自混合效应》（Bate 等，《Nature Communications》，13卷，6573页，2022年）中所用的实验数据。本数据库包含两类数据集：(1) 悬浮于活性-非活性流体系统（active-inactive fluid systems）中的示踪粒子（tracer particles）成像数据；(2) 悬浮于活性-非活性流体系统中的荧光素（fluorescein）成像数据。两类数据集均配套了用于演示从数据中提取混合相关参数的分析脚本，相关参数包括界面演化指数、界面演化系数与混合时间。

第一类数据集包含4个文件：Data1.zip、analysis1.m、Mask.tif与Tracking.mat。其中Data1.zip收录了论文图2c中[cATP]扫描组单次实验的1小时逐帧示踪粒子数据，对应样品使用5 mM笼状ATP（caged ATP）。示踪粒子的追踪采用Ouellette等人开发的追踪软件完成（《Experiments in Fluids》，40卷，301-313页，2005年），追踪结果输出至Tracking.mat，可通过运行analysis1.m导入至MATLAB（需搭配MATLAB曲线拟合工具箱（Curve Fitting Toolbox）、图像处理工具箱（Image Processing Toolbox）与并行计算工具箱（Parallel Computing Toolbox））。该脚本可输出本数据集的界面演化指数与界面演化系数，同时支持对Mask.tif中标记为黑色像素的特定感兴趣区域进行分析，本次分析中将感兴趣区域设置为整幅图像。

第二类数据集包含2个文件：Data2.zip与analysis2.m。Data2.zip收录了论文图5中荧光素扫描组单次实验的1小时逐帧荧光素成像数据，对应样品包含3.2 mM笼状ATP、3 μM笼状荧光素、80 nM K365二聚体，样品池高度为100 μm。本实验旨在探究活性流体系统中悬浮荧光素的混合时间。如需提取混合时间，需先解压Data2.zip，再在MATLAB中运行analysis2.m（需搭配MATLAB曲线拟合工具箱与图像处理工具箱），该脚本将读取荧光素图像以分析多尺度范数，并输出混合时间。