Protein-protein interaction network dynamics across environments by DNA barcode sequencing

We have developed to quantitatively assay protein-protein interactions (PPIs) by barcode sequencing (Schlecht et al., Nature Comm. 2017, 8: 1-9). Here, we scale up this technology to quantify changes in relative in vivo PPI abundance of 1.6 million protein pairs across 9 growth conditions, with replication, for a total of 44 million measurements. To the best of our knowledge, this is the first large-scale study to move beyond a static view of the protein interactome by examining how PPI networks change across environments.