mRNA and lncRNA profiling of mice spleens inoculated with group ACYW135 meningococcal polysaccharide vaccine

Group ACYW135 meningococcal polysaccharide vaccine (MPV-ACYW135) is a classical common vaccine used to prevent Neisseria meningitidis serogroup A, C, Y, W135. Although immunological research on MPV has been relatively complete in the past few decades, studies on the vaccine at the transcriptional levels are still limited. In the present study, mRNAs and lncRNAs related to immunity were screened from spleens of mice inoculated with MPV-ACYW135 and compared with the control group to identify the effect and mechanism of these mRNAs and lncRNAs on the immune response. A comprehensive analysis of mRNAs and lncRNAs between MPV group and control group was performed by RNA-seq with bioinformatics. Overall design: The experiment was divided into 2 groups with 3 mice in each group. Mice in one group were injected with MPV-ACYW135, and the other group was the control group. The spleen is used for RNA sequencing. Comparative gene expression profiling analysis of RNA-seq data for MPV group (MPV_S_1, MPV_S_2, and MPV_S_3) and control group (Control_S_1, Control_S_2, and Control_S_3).