SARS-CoV-2-spec. human bone marrow plasma cells _new dataset

Determination of frequencies of SARS-CoV-2 Spike/RBD/Nucleocapsid(NP)-specific plasma cells and their subsets in human bone marrow. Conclusion: Our results support the early formation of SARS-CoV-2-reactive CD19+ and CD19- presumably long-lived plasma cells in the human BM after repeated vaccination against SARS-CoV-2, as an important prerequisite for durable serological immunity preventing severe courses of COVID-19. Compensation controls with single stained cells. Verification of the specificity of the antigen-specific B cell staining (Tetramer staining) by immunized/non-immunized PBMC controls and unloaded (Streptavidin only) controls.