Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R2_LG261.xlsx

A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels

将A549 Xrn1基因敲除细胞分别予以空白感染、野生型甲型流感病毒A/Puerto Rico/8/1934 (H1N1)（简称"PR8"）感染，以及PA-X缺陷型PR8-PA(∆X)感染，感染16小时后提取总RNA。采用qRT-PCR定量检测甲型流感病毒HA基因与PA-X靶标人G6PD基因的mRNA水平，并以18S rRNA水平作为内参进行归一化处理。