Additional file 1: of NLRP3 promotes tumor growth and metastasis in human oral squamous cell carcinoma

Table S1. IHC Staining score of the expression levels of NLRP3 and IL-1β in 77 OSCC tissues. The positive grade was determined based on the Immuno-Reactive-Score (IRS), which was calculated by multiplying staining proportion score (PS) and staining intensity score (IS). PS was classified as 0 (0%), 1 (1%–25%), 2 (26%–50%), 3 (51%–75%), and 4 (76%–100%). IS was classified as 0 (negative), 1 (weak), 2 (moderate) and 3 (strong). The final staining score was calculated by multiplying IS by PS. According to the final score, patients with different expression were divided into three groups: negative expression group (IRS = 0), weak expression group (IRS = 1–3) and strong positive expression group (IRS = 4–12). (XLSX 15 kb)

表S1. 77例口腔鳞状细胞癌（Oral Squamous Cell Carcinoma, OSCC）组织中NLRP3与IL-1β（Interleukin-1β）表达水平的免疫组化（Immunohistochemistry, IHC）染色评分。阳性等级依据免疫反应评分（Immuno-Reactive-Score, IRS）判定，该评分由染色比例评分（staining proportion score, PS）与染色强度评分（staining intensity score, IS）相乘计算得到。其中PS划分为0（0%）、1（1%~25%）、2（26%~50%）、3（51%~75%）、4（76%~100%）五个等级；IS划分为0（阴性）、1（弱）、2（中等）、3（强）四个等级。最终染色评分为IS与PS的乘积。根据该最终评分，将不同表达水平的患者分为三组：阴性表达组（IRS=0）、弱阳性表达组（IRS=1~3）及强阳性表达组（IRS=4~12）。（XLSX 15 kb）