Microscopy image data of a titration series with DNA surface strands and condensate-forming DNA nanomotifs

The dataset contains microscopy image data of DNA-nanomotifs (color channel 2) that can form liquid-like condensates, especially in association with target DNA strands that can act as condensation surfaces (color channel 1). The data were recorded on a VisiTech iSIM microscopy with a 40X water immersion objective (Apo LWD 40x WI λS DIC N2, NA 1.15). Images were recorded using dual ORCA-Flash4.0 V3 cameras with simultaneous two-channel acquisition to avoid displacement of objects due to consecutive acquisition of color channels. The file names contain the flow channel number, the presence of condensation surfaces (SX4), and the amount of added nanomotif solution in microliters (NX in µl).

The image files are in the .nd2 Nikon proprietary format, but can be read with the OME bioformats libraries.

Scripts for the analysis of these data are available via a GitHub repository: https://github.com/lhilbert/SurfacesAndCondensates

This repository contains data recorded on 13 March, 2023

The file size of data were too large, so additional repositories were opened. The complete date need to be also downloaded from these additional repositories:

EXTRA DATA 1/2: 10.5281/zenodo.13788608 

https://doi.org/10.5281/zenodo.13788608

EXTRA DATA 2/2: 10.5281/zenodo.13788711

https://doi.org/10.5281/zenodo.13788711

There is another data set that contains a repeat of the same conditions, recorded on 10 March, 2023