Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (TREx Und 12h 24hInd ChIP-Seq). Human gammaherpesvirus 8

The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation Overall design: KSHV infected, TRExBCBL1-RTA or iSLKBac16WT cells were induced with doxycycline for 12h or 24h to trigger the lytic reactivation, cells were crosslinked and subjected for chromatin isolation and sonication. ChIP assay was performed using specific antibodies.