microRNA expression profiles in far-infrared treated human endothelial precursor cells

High glucose impairs the angiogenic activities of late endothelial precursor cells (EPC). We found that far infrared (FIR) treatment restored partially the activity of late EPC. However, the mechanisms are unclear. We applied microRNA expression microarrays to assess the microRNA expression profiles of high glucose-treated late EPC with or without FIR treatment. Late EPCs isolated from peripheral blood were cultured in high glucose condition for 48 hours, and then treated with or without FIR radiation for 30 minutes. Total RNA were extracted and evaluated with the Agilent Human miRNA Oligo Microarray R14 V2 chips.