Omics-Lethal Human Viruses, MERS-CoV Experiment MCL001

The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (icMERS) and icMERS-CoV mutants icMERS-CoV-RFP, icMERS-CoV-dNSP16, icMERS-CoV-d4B, and icMERS-CoV-d3 virus infection. Sample data was obtained from human bronchial epithelial cells (Calu-3 clone 2B4) for mRNA and miRNA transcriptome expression analysis. Secondary host-associated viral dataset downloads contain one or more statistically processed (normalization data transformation) quantitative dataset collections resulting in qualitative expression analyses of primary host-pathogen experimental study designs. Transcriptomics dataset downloads each have a direct relationship to a primary sample submission corresponding to a specific MERS-CoV virus infection.

本实验旨在评估人类宿主对野生型中东呼吸综合征冠状病毒（icMERS）以及icMERS-CoV突变体icMERS-CoV-RFP、icMERS-CoV-dNSP16、icMERS-CoV-d4B和icMERS-CoV-d3感染的宿主应答情况。实验采集人支气管上皮细胞（Calu-3克隆2B4）的样本，用于mRNA与miRNA转录组的表达分析。二级宿主相关病毒数据集下载资源包含经统计处理（含数据归一化与转换）的定量数据集合集，可用于开展针对宿主-病原体原始实验研究设计的定性表达分析。所有转录组学数据集下载项，均与对应特定MERS-CoV感染的原始样本提交数据存在直接关联。