Separation, Concentration and Determination of Trace Thiamphenicol in Egg, Milk and Honey Using Polyoxyethylene Lauryl Ether-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography

As a pretreatment technique to trace analysis, polyoxyethylene lauryl ether (POELE10)- salt aqueous two-phase extraction system (ATPES) is coupled with high performance liquid chromatography to analyse thiamphenicol (TAP) in egg, milk and honey. The analysis of the phase behaviors of the ATPES found that the extraction efficiency and enrichment factor of TAP was influenced by the types of salts, the concentration of salt, the concentration of POELE10, the concentration of 1-propanol and the temperature. The optimized conditions of experiment were determined in the multi-factor experiment by using response surface methodology. The extraction efficiency of TAP is up to 99.59% and the optimal values of enrichment factor of TAP was 27 under the optimized conditions. The limit of detection and the limit of quantification of TAP are 0.5 µg kg−1 and 1.5 µg kg−1, which meet the needs of determination of trace or ultratrace TAP in food.

作为痕量分析的前处理技术，将月桂醇聚氧乙烯醚（POELE10）-盐双水相萃取体系（ATPES）与高效液相色谱法联用，对鸡蛋、牛奶与蜂蜜中的甲砜霉素（TAP）进行检测分析。对该双水相萃取体系的相行为研究表明，甲砜霉素的萃取效率与富集因子受盐种类、盐浓度、月桂醇聚氧乙烯醚（POELE10）浓度、1-丙醇浓度及温度的影响。本研究通过响应面法开展多因素实验，确定了体系的最优实验参数。在最优条件下，甲砜霉素的萃取效率可达99.59%，其富集因子最优值为27。该方法中甲砜霉素的检出限为0.5 μg·kg⁻¹，定量限为1.5 μg·kg⁻¹，可满足食品中痕量乃至超痕量甲砜霉素的检测需求。