HMGB1 and Alu-siRNA inhibit the growth of human lung tumor cells

We performed expression profiling using microarray technology. The expression profiling identified genes or mechanism potentially regulating the proliferation of human lung cancer cells by BoxA of HMGB1 and Alu-siRNA transfection. The RNAs of our experiment were hybridized with Agilent SurePrint G3 Human GE 8X60K, V3 Microarrays (Agilent®). Two type of human lung cancer cell lines (H1650 and H1975) and embryonic kidney cell line (HEK-293) were transfected with BoxA of HMGB1 and siRNA-Alu, plasmid control and mock were represented internal control. The cells were harvested after transfection for 4 hour.