ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293.sgd

Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.

将Xrn1基因敲除的A549细胞分别感染野生型A/波多黎各/8/1984（H1N1）（简称PR8）、PA-X缺陷型PR8-PA(∆X)病毒，或进行模拟感染，于感染16小时后开展RNA提取。随后采用针对BCAP31或SLC7A5的特异性引物进行5'末端快速扩增技术（5' RACE），以区分含内含子与不含内含子的条带。将聚合酶链式反应（PCR）产物进行琼脂糖凝胶电泳。