TwinNetworkDataset004

The data comprises bright-field time-lapse images of zebrafish embryos acquired in multiple batches within multi-well plates using an Acquifer Imaging Machine. Embryos from incrosses of sqt+/-, crosses of Tg(sebox:EGFP) and sqt+/-, and crosses of Tg(sebox:EGFP) and Tg(gsc:TurboRFP) zebrafish strains were used for imaging. Imaging started at 4 hpf and ended at 22.5 hpf. Images of embryos were acquired at 134 acquisition time points with a time interval of 498 s between each acquisition time point. Individual embryo segments were identified and extracted using a trained neural network for object detection. Within this experiment folder, data are organized by microscope position and embryo number.

本数据集包含采用Acquifer成像系统（Acquifer Imaging Machine）于多孔板内多批次采集的斑马鱼胚胎明场延时成像数据。成像所用胚胎来自三类交配组合：sqt+/-品系自交、Tg(sebox:EGFP)与sqt+/-品系杂交，以及Tg(sebox:EGFP)与Tg(gsc:TurboRFP)品系杂交。成像时段为4 hpf至22.5 hpf，共获取134个时间点的胚胎图像，各时间点间的采集间隔为498秒。采用经训练的目标检测神经网络识别并提取单个胚胎图像区块。本实验文件夹内的数据按照显微镜拍摄位置及胚胎编号进行有序组织。