Soil fungal community Raw sequence reads under GO

Graphene oxide (GO) has been suggested as an efficient assistant additive to eliminate non-specific amplification of the polymerase chain reaction (PCR). Although many studies have focused on exploring its molecular mechanism, the effects of GO on quantitation of microbial community have not been explored yet. In this study, GO was added in PCR system to explore the changes on removing typical amplification errors, such as chimera and mismatches on two kinds of mock communities (an even and a staggered mock communities) and environmental samples.