Summary table of gut content DNA sequencing

The table presents the number of sequences per OTU and per sample (fish gut). Sequence clustering was done using CROP. OTUs were identified from BLASTn searches performed in the Moorea BIOCODE database and GENBANK. We provide BIOCODE specimen number and/or GenBank accession number in the table whenever sequence similarity to a reference barcode was >98%. Additional information on BIOCODE specimen can be obtained from http://biocode.berkeley.edu/. When sequence similarity was <98%, we used the Bayesian assignment tool implemented in SAP to assign each OTU to a higher taxonomic group, accepting assignments at a significance level of 95% (posterior probability). Note that the representative sequence for each OTU is provided as a supplementary file in the manuscript.

本表格展示了每个操作分类单元（Operational Taxonomic Unit, OTU）以及每个鱼类肠道样本的序列条数。序列聚类分析采用CROP工具完成。OTU的鉴定通过在莫雷阿生物编码（Moorea BIOCODE）数据库与GenBank数据库中开展BLASTn比对搜索实现。当待比对序列与参考条形码的序列相似度超过98%时，本表格将提供对应的BIOCODE标本编号及/或GenBank登录号。若需获取BIOCODE标本的更多相关信息，可访问网址http://biocode.berkeley.edu/。当序列相似度低于98%时，我们采用SAP内置的贝叶斯分类工具，将每个OTU归类至更高阶的分类学类群，分类判定的显著性水平设定为95%（后验概率）。请注意，本研究手稿的补充文件中提供了每个OTU的代表性序列。