Transfection experiments with several innate-immune stimulators and pathogens in human cells

The innate immune system represents the first line of defense against invading organisms. Among several key innate-immune signaling pathways, the cGAS-STING signaling has emerged as a pivotal component of innate immunity for both foreign- and self-DNA detection. Here, we conducted both computional and experimental assays to identify and characterize novel and key innate immune protein-coding genes and long noncoding RNAs Total RNA after transfecting with innate-immune stimulators and pathogens were isolated and sequenced by deep sequencing using Illumina HiSeq 4000