FOXM1 reversion of aging chromatin accessibility profiles and TEAD4/FOXM1/RHNO1 cis-regulatory elements. FOXM1 reversion of aging chromatin accessibility profiles and TEAD4/FOXM1/RHNO1 cis-regulatory elements

Using ATAC-seq, we profiled chromatin accessibility in human dermal fibroblasts (HDFs) from individuals with ages ranging from neonatal to octogenarian. Combining with 4C-seq, we further found that TEAD4, FOXM1 and RHNO1 share a conserved transcriptional regulatory landscape controlled by a novel and age-dependent enhancer that loses accessibility with aging and whose deletion drives senescence. Finally, we found that FOXM1 ectopic expression in elderly cells partially resets chromatin accessibility to a youthful state.