Profiling expression of genes at a critical developmental timepoint in diaphragm development.

Purpose: The goal of this study is to determine what genes are expressed, and at what level, in the developing diaphragm at a timepoint shown to be critical in the initiation of congenital diaphragmatic hernias in mice. Methods: PPFs were dissected from staged mouse embryos Methods: summary Purpose: The goal of this study is to determine what genes are expressed, and at what level, in the developing diaphragm at a timepoint shown to be critical in the initiation of congenital diaphragmatic hernias in mice. Methods: PPFs were dissected from staged mouse embryos Overall design: Epression profile of 2 biological pooled E12.5 pleuroperitineal fold pairs (2 dissected pairs per biological replicate) to prioritize candidate genes implicated from human congenital diaphragmatic studies.