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TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA629015
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资源简介:
We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.
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2020-04-28
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