<b>Roles of a Y-linked iDmrt1 paralogue and Insulin-Like Androgenic Gland Hormone in Sexual Development in the Tropical Rock Lobster, </b><b><i>Panulirus ornatus</i></b>

This study investigates the functions of the Y-linked <i>iDmrt1</i> paralogue, <i>Po-iDMY</i> and insulin-like androgenic gland hormone (<i>Po-IAG</i>) in the process of sexual development in the tropical rock lobster, <i>Panulirus ornatus</i> (TRL). Previously, we identified that <i>Po-iDMY</i>, a male-specific heterogametic (Y-linked) paralogue of the autosomal <i>Po-iDmrt1 </i>found in TRL, is a second sex-linked <i>iDmrt</i> gene identified in invertebrates. Using 5’ and 3’ rapid amplification of cDNA ends and data from a draft male genome (with an assembly genome size of approximately 2.446 Gbp and 87% BUSCO completeness), we obtained the full-length <i>Po-iDMY</i> gene (coding for a 312 amino acids protein), which includes a 411 bp male-specific sequence located at the 3’ untranslated region of <i>Po-iDMY</i> mRNA used as a sex marker. Notably, this sex marker sequence is reported for the first time in our genome analysis. However, <i>Po-iDMY</i> is not expressed across various tissues and developmental stages across embryos, juveniles and adults. This observation suggests that Po-iDMY may not tightly regulate Po-IAG in this species, indicating that silencing <i>Po-IAG</i> could potentially induce sex change. Further expression analyses confirmed that <i>Po-IAG</i> expression is not male-specific. Despite this, <i>Po-IAG</i> silencing at an early juvenile stage generated two potential neo-females, implying that sexual manipulation could be a promising technique in TRL.

本研究针对热带岩龙虾（*Panulirus ornatus*，简称TRL）的性别发育过程，探究了Y连锁的*iDmrt1*旁系同源基因*Po-iDMY*与胰岛素样雄性腺激素（insulin-like androgenic gland hormone, *Po-IAG*）的功能。此前本团队已鉴定出*Po-iDMY*：该基因是热带岩龙虾中常染色体*Po-iDmrt1*的雄性特异性异配（Y连锁）旁系同源基因，也是无脊椎动物中发现的第二个性连锁*iDmrt*基因。本研究通过5'与3' cDNA末端快速扩增技术（rapid amplification of cDNA ends, RACE），结合组装基因组大小约2.446 Gbp、BUSCO完整度达87%的雄性参考基因组草案数据，成功获得*Po-iDMY*基因的全长序列：其编码的蛋白质包含312个氨基酸，且在*Po-iDMY* mRNA的3'非翻译区中存在一段411 bp的雄性特异性序列，可作为性别分子标记。值得注意的是，该性别标记序列在本次基因组分析中为首次报道。然而，*Po-iDMY*在胚胎、幼体与成体的各类组织及发育阶段中均未检测到表达。该结果提示，*Po-iDMY*可能并未对*Po-IAG*实现紧密调控，这意味着沉默*Po-IAG*或可诱导该物种发生性别转换。进一步的表达分析证实，*Po-IAG*的表达并非雄性特异性的。尽管如此，在幼体早期阶段沉默*Po-IAG*，成功获得了2只潜在的新雌体（neo-females），这表明性别调控技术在热带岩龙虾中极具应用前景。

搜集汇总

背景与挑战

背景概述

该数据集包含热带石龙虾（Panulirus ornatus）的基因组和分子数据，用于研究Y连锁的iDmrt1旁系同源基因（Po-iDMY）和胰岛素样雄性腺激素（Po-IAG）在性发育中的作用。研究发现Po-iDMY包含一个首次报道的性别标记序列，但表达分析显示其不广泛表达，而Po-IAG沉默可能诱导性别转变，为龙虾的性别操控提供了技术潜力。数据集支持相关科学分析，并遵循CC BY 4.0许可协议。