Application of XeCl308nm excimer laser radiation to mutate industrial microorganisms

In this study, we have investigated the effects of an XeCl308 nm excimer laser radiation on bacterial mutagenesis. Our experiments have revealed that the mutagenesis inducted by the XeCl308 nm excimer laser radiation is independent from RecA protein, the regulator of the SOS response, unlike UV254 nm radiation that is not mutagenic for Escherichia coli mutants lacking the RecA protein. This found suggests that the UV308 nm laser radiation might be mutagenic also in microorganisms naturally lacking the SOS response. To test this hypothesis, we applied our innovative mutagenesis approach on Nonomuraea ATCC 39727, an industrial strain producing an antibiotic, which is relatively refractory to UV254 nm radiation-induced mutagenesis. Our results demonstrated the efficiency of XeCl308 nm excimer laser radiation to induce mutagenesis in Nonomuraea ATCC 39727.

本研究探究了氯化氙(XeCl)308nm准分子激光辐射对细菌诱变的影响。实验结果表明，氯化氙308nm准分子激光辐射诱导的诱变效应不依赖于SOS应答(SOS response)的调控蛋白RecA(RecA)；与之不同的是，254nm紫外(UV254nm)辐射对缺失RecA蛋白的大肠杆菌(Escherichia coli)突变体并无诱变活性。这一发现提示，308nm紫外激光辐射在天然缺乏SOS应答的微生物中同样可能具备诱变活性。为验证该假说，我们采用创新性诱变方法处理野野村菌(Nonomuraea) ATCC 39727：该菌株为可生产抗生素的工业菌种，对254nm紫外辐射诱导的诱变相对难以发生。本研究结果证实，氯化氙308nm准分子激光辐射可有效诱导野野村菌ATCC 39727产生诱变效应。