A Human Brain Map of Mitochondrial Respiratory Capacity and Diversity

Supplementary Video 1 | Human brain slab voxelization using a computer numerical control (CNC) cutter. Milling was done over ~5h at -25°C (freezer room) with routing of the drill bit controlled by a computer in the next room (+4°C).

Supplementary File 1 | Extended discussion of the loadings for MRI-based metrics and mitochondria biology from Extended Table 1.

Source Data 1 | Analysis of snRNAseq data. Detailed markdown of the single nucleus RNA sequencing (snRNAseq) analysis, including the code for data pre-processing, visualization, cluster identification and mitochondrial analysis. This file also shows additional figures supporting the analysis. An HTML file also available at http://humanmitobrainmap.bcblab.com.

Source Data 2 | A complete dataset of mitochondrial parameters (CI-IV activities, MitoD, TRC and MRC) and their correlations with MRI and PET metrics. An Excel spreadsheet with MRI and PET values for each voxel.

Source Data 3 | Software for the voxelization of the tissue. Igor Pro procedure file for 1) defining the parameters to clean and partition a frozen brain slab, 2) generating G-codes used to control the CNC router, and 3) randomizing samples and assigning them to specific assay plates.

Source Data 4 | Software for the voxelization of the tissue. Igor Pro procedure file for 1) derandomizing the samples after the assays are complete, and 2) plotting the assay data on the topological map of the brain.

Source Data 5 | Morphing the image of the brain onto MNI space coordinates. Python code used for the conversion of the image of the brain slab to Neuroimaging Informatics Technology Initiative (NIfTI) format.

补充视频1 | 采用计算机数控（Computer Numerical Control, CNC）刀具完成的人脑切片体素化操作。整个铣削流程在-25℃的低温冷库中耗时约5小时，钻头的运动路径由隔壁4℃房间内的计算机控制。

补充文件1 | 针对扩展表1中基于磁共振成像（Magnetic Resonance Imaging, MRI）指标与线粒体生物学相关载荷的拓展讨论。

源数据1 | 单细胞核RNA测序（single nucleus RNA sequencing, snRNAseq）分析的详细Markdown文档，包含数据预处理、可视化、聚类鉴定以及线粒体分析的代码。该文件还附带了支撑本次分析的补充图表，相关HTML文件可通过http://humanmitobrainmap.bcblab.com获取。

源数据2 | 线粒体参数（复合物I-IV活性、MitoD、TRC及线粒体呼吸链复合体（Mitochondrial Respiratory Chain, MRC））的完整数据集，及其与MRI和正电子发射断层扫描（Positron Emission Tomography, PET）指标的相关性数据。本文件为Excel表格，包含每个体素对应的MRI与PET检测值。

源数据3 | 脑组织体素化处理软件。为Igor Pro程序文件，可实现三项功能：1）定义参数以清洗并分割冷冻脑切片；2）生成用于控制CNC雕刻机的G代码；3）完成样本随机化并分配至指定检测板。

源数据4 | 脑组织体素化处理软件。为Igor Pro程序文件，可实现两项功能：1）检测完成后对样本进行去随机化处理；2）将检测数据绘制于大脑拓扑图谱之上。

源数据5 | 大脑图像配准至MNI空间坐标流程。用于将脑切片图像转换为神经影像信息技术倡议（Neuroimaging Informatics Technology Initiative, NIfTI）格式的Python代码。