Effects of macauba cake on profile of rumen protozoa of lambs

ABSTRACT The present study was conducted to determine the effects of the inclusion of macauba cake, from biodiesel processing, on profile of rumen protozoa of Santa Ines lambs. Twenty-four lambs were randomized in block design supplemented with macauba cake at 0, 100, 200, and 300 g/kg of dry weight of the diet. Concentrations of small, medium, and large protozoa had quadratic relationships with inclusion of macauba cake, with maximum protozoa occurring at 100 g/kg. High genus diversity occurred in rumen fluid of lambs that did not feed macauba cake, comprising 13 protozoa genera. However, only the genera Isotricha, Charonina, Entodinium, Diplodinium, Eodinium, Diploplastron, and Polyplastron were detected in lambs fed 300 g/kg macauba cake, indicating that these protozoa were resistant to the effects of the cake. Addition of macauba cake levels greater than 100 g/kg show antiprotozoal effect in the rumen.

摘要
本研究旨在探究生物柴油加工副产物莫卡乌巴饼（macauba cake）的添加水平对圣伊内斯羔羊（Santa Ines lambs）瘤胃原虫群落组成的影响。本试验选取24只羔羊，采用随机区组设计，以日粮干物质为基础，分别添加0、100、200及300 g/kg的莫卡乌巴饼作为补充饲料。小型、中型与大型原虫的丰度与莫卡乌巴饼的添加量呈二次曲线相关，当添加量为100 g/kg时原虫丰度达到峰值。未饲喂莫卡乌巴饼的羔羊瘤胃液中原虫属多样性较高，共包含13个原虫属。然而，饲喂300 g/kg莫卡乌巴饼的羔羊瘤胃液中仅检出等毛虫属（Isotricha）、查氏虫属（Charonina）、内毛虫属（Entodinium）、双毛虫属（Diplodinium）、曙虫属（Eodinium）、双前毛虫属（Diploplastron）以及多毛虫属（Polyplastron），表明这些原虫对该饼粕的干预效应具有抗性。当莫卡乌巴饼添加量超过100 g/kg时，可在羔羊瘤胃中发挥抗原虫作用。