CYP450-Mediated Detoxification and NF-?B/NLRP3-Driven Mechanisms in Emodin-Induced Hepatotoxicity in vitro and in vivo

Emodin, a bioactive compound abundant in Chinese medicinal herbs, possesses broad therapeutic potential but raises safety concerns due to conflicting reports of hepatotoxicity. This study investigated the toxicological mechanisms underlying emodin-induced liver injury. We showed that emodin induced dose-dependent cytotoxicity and hepatic injury in HepG2 cells. Moreover, emodin (400 mg/kg) induced hepatotoxicity in mice after administered for three weeks.This study delineates the metabolic detoxification pathways of emodin and elucidates its toxicological mechanisms. Overall design: RNA sequencing was performed on liver tissue from wild-type C57BL/6 mice with and without emodin.