Revealing Missing Human Protein Isoforms Based on Ab Initio Prediction, RNA-seq and Proteomics

In this project, we design a methods to detect novel transcripts integrating ab initio prediction on genomic sequences and filtration with various RNA-seq data. 100 splice sites were selected and RT-PCR on 48 human tissues and cell lines (including a blank negative control) were conducted. The product of the same tissue or cell line were mixed and sequenced with Illumina Mi-seq.

本研究设计了一种整合基因组序列从头预测（ab initio prediction）与多源RNA-seq数据过滤的新转录本检测方法。研究团队选取100个剪接位点（splice sites），针对48种人体组织及细胞系（包含空白阴性对照）开展逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction，RT-PCR）实验；将同一组织或细胞系的扩增产物混合后，采用Illumina MiSeq测序平台进行测序。