Expression data from catalase stably transfected A375 human melanoma cells. Homo sapiens

Reactive oxygen species (ROS) are implicated in tumor transformation by modulating proteins involved in differentiation, proliferation and invasion. In order to identify genes that may support melanoma progression or regression after an antioxidant system (AOS) response, we developed and characterized a human melanoma cell model with different levels of ROS by stably overexpressing the antioxidant enzyme catalase in A375 amelanotic melanoma cells, and whole genome gene expression patterns were analyzed by microarrays. We used gene expression microarrays to study the AOS global response to catalase overexpression and to identify up-regulated and down-regulated genes during progression or regression of melanoma. Overall design: Gene expression microarrays were performed in A375 amelanotic melanoma cells stably transfected with human catalase cDNA. A375-A7 and A375-G10 cell clones were obtained by clonal dilution. A375-PCDNA3 (transfected with empty vector) and A375 cells were used as controls.