Spatial transcriptomic sequencing of repaired lung transplanted with human P63+ progenitor [STOmics-seq]

KRT5+P63+ progenitor cells located in the basal layer of the airway epithelium have been identified as a promising candidate for lung epithelium repair. Such cells are originated from primitive progenitors in distal airways and could expand/migrate to inflamed damaged lung parenchymal region to form ‘saccule’. To further investigate the characteristics and potential function of the saccule structure, we performed the high-resolution spatial transcriptomic analysis on the 35 dpt lung section. We isolated the human P63+ progenitors and transplanted the GFP-labelled P63+ progenitors into the bleomycin-injured mouse lung by intratracheal instillation. Mice were sacrificed at 35 days post transplantation and their lung tissue were harvest to detect GFP signal by fluorescence stereomicroscope for Stereo-seq.