Expression data from mouse precision cut lung slices (PCLS) after human rhinovirus (RV) infection and after treatment with bronchobini (BRO).

Transcriptome analysis revealed a robust and multifactorial mode of action of BRO. BRO suppressed RV induced responses, e.g. upregulation of the antiviral interferon (IFN) signaling pathway, while stimulating this pathway under baseline conditions. Suppression of RV-induced cytokine release and downregulation of IFN signaling pathway confirmed that BRO exerts a beneficial anti-inflammatory effect during viral infection. Mouse PCLS containing airways were prepared from BALB/c mice. For each experiment PCLS from three mices were pooled. Mouse PCLS were pre-incubated with BRO in three different doses or BRO vehicle. Afterwards mPCLS were infected with RV, replicative deficient UV-RV or medium as sham infection. mPCLS samples were used for RNA isolation and gene expression analysis.