RT2 Profiler Array Data for Adventitia and PVAT at 8 and 24 weeks on control and high-fat diet

The RT² Profiler Array uses a Microsoft Excel-based file that allows for import of C_T values from the real time PCR machine. Data were gathered in separate Excel sheets for each of the following four comparison: 8 week Adventitia HF vs Control; 8 week PVAT HF vs Control; 24 week Adventitia HF vs Control; and 24 week PVAT HF vs Control. The threshold value for each plate in this study was set to 0.16. In each sheet, C_T values were compared to the average of five (5) different reference genes: b-actin (Actb), beta-2-microglobulin (B2M), hypoxanthine phosphoribosyltransferase 1 (Hprt1), lactate dehydrogenase A (Ldha) and Ribosomal protein, large, P1 (Rplp1). The 2^-^D^CT were calculated for the gene of interest where DC_Tis [C_Tvalue of the gene of interest – C_T value of averaged house keeping gene]. From this, the fold change of HF/control was calculated. These are the values reported in Supplemental Table 1. A fold change of 2.00 or above was considered biologically meaningful. Statistically, the p values were calculated based on a Student’s t-test of the replicate 2^-^D^C^T values for each gene in the Control group and Treatment (HF) groups.

RT² Profiler Array采用基于Microsoft Excel的文件，可导入实时荧光定量PCR（real-time PCR）仪的C_T值。本研究针对以下四组对照分别在独立的Excel工作表中收集数据：8周外膜心力衰竭组与对照组、8周血管周围脂肪组织心力衰竭组与对照组、24周外膜心力衰竭组与对照组以及24周血管周围脂肪组织心力衰竭组与对照组。本研究中每块反应板的阈值设定为0.16。在每张工作表中，将C_T值与5种持家基因（house keeping gene）的平均值进行比对：β-肌动蛋白（b-actin，Actb）、β2-微球蛋白（beta-2-microglobulin，B2M）、次黄嘌呤磷酸核糖基转移酶1（hypoxanthine phosphoribosyltransferase 1，Hprt1）、乳酸脱氢酶A（lactate dehydrogenase A，Ldha）以及核糖体蛋白大亚基P1（Ribosomal protein, large, P1，Rplp1）。针对目的基因计算2^-ΔC_T值，其中ΔC_T = [目的基因的C_T值 - 持家基因平均C_T值]。据此计算心力衰竭组（HF）与对照组的倍数变化，该数值即补充表1中所报告的数据。倍数变化≥2.00被认为具有生物学意义。统计学分析方面，针对对照组与治疗组（HF组）中每个基因的重复测定2^-ΔC_T值，采用学生t检验（Student’s t-test）计算p值。