Figure S1 - Next-Generation cDNA Screening for Oncogene and Resistance Phenotypes
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Full length-att method for library construction. A. cDNA was generated from PC9 and K562 cells using the In-Fusion SMARTer Kit and using the full length-att method, which modifies the former approach using the attB1_forward and attB2_reverse primers for first strand synthesis. cDNA was separated by agarose gel electrophoresis. λ indicates size ladder. B. Size fractionation of cDNA from PC9 and K562 cells generated using the full length-att method. C. BsrG1 digest of pDONR222 plasmids harboring cDNA from the K562 library generated using the full length-att method. D. Comparison between methodologies. Negative and positive controls are from the CloneMiner II kit (Invitrogen). The PC9ER library is described in Figure 3. CFUs indicates colony forming units. (EPS)
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2015-12-02
