Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse epididymis (jrc_mus-epididymis -2)

Sample: Wild-type, 6 months old mouse, strain: C57BL/6 from Jackson LabSample Description: A dataset featuring isotropic 3D electron microscopy data of the mouse epididymis (jrc_mus-epididymis-2). The sample was obtained from a 6-month-old, wild-type C57BL/6 mouse strain sourced from Jackson Laboratory. Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After room temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven. The sample was provided by Wei-Ping Li (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia). This dataset offers a comprehensive view of the mouse epididymis' cellular architecture and facilitates further research in reproductive biology.Protocol: Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After room temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven.Contributions: Sample provided by Wei-Ping Li and Zhiyuan Lu (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).Acquisition ID: jrc_mus-epididymis -2Voxel size (nm): 4.0 x 4.0 x 4.0 (X, Y, Z)Data dimensions (µm): 20.9 x 20.5 x 7.0 (X, Y, Z)Imaging start date: 2022-07-14Dataset URL (Redirect): s3://janelia-cosem-datasets/jrc_mus-epididymis-2/jrc_mus-epididymis-2.zarr/recon-1/em/Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-epididymis-2Imaging duration (days): 4Landing energy (eV): 1Imaging current (nA): 0.25Scanning speed (MHz): 0.200