Interaction of Anabolic Androgenic Steroids and Induced Tooth Movement in Rats

Abstract This study evaluated the interaction between tooth movement and two anabolic androgenic steroids (AAS), Deposteron® and Nebido®. One hundred Wistar rats were divided into 3 groups: control (C) n=30, Nebido experimental (N) n=35 and Deposteron experimental (D) n=35. The control group was subdivided into 6 subgroups: 1, 2, 3, 5, 7 and 14. The experimental groups were subdivided into 7 subgroups: 0, 1, 2, 3, 5, 7 and 14, which corresponded to the day of animal’s euthanasia after applying orthodontic force. Orthodontic devices were used to induce tooth movement using 50 cN of reciprocal force between the maxillary right first molar and the maxillary incisors. After euthanasia, the tissues were processed and stained with hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP). Osteoclasts, Howship’s lacunae and blood vessels were quantified. Groups N and D showed acceleration in the reorganization of the periodontal ligament compared to group C. The peak of the histological events occurred in group C on day 5 and in groups N and D on day 3 after installation of the orthodontic device. There was a statistically significant difference in the number of osteoclasts (p<0.05) between groups N3 and C3, and between groups N3 and D3. Supra-physiological doses of the AAS Nebido® and Deposteron® altered the number of osteoclasts, Howship’s lacunae and blood vessels, accelerating the reorganization of the periodontal ligament, resulting in accelerated biological effects from the induced tooth movement in rats.

摘要 本研究评估了牙齿移动与两种合成代谢雄激素类固醇（anabolic androgenic steroids, AAS）——Deposteron®与Nebido®之间的相互作用。实验选用100只Wistar大鼠，分为3组：对照组（C组）n=30、Nebido®实验组（N组）n=35、Deposteron®实验组（D组）n=35。对照组进一步分为6个亚组，对应安乐死天数分别为1、2、3、5、7、14天；实验组则分为7个亚组，对应施加正畸力后安乐死的天数分别为0、1、2、3、5、7、14天。本研究通过在上颌右侧第一磨牙与上颌切牙之间施加50cN的交互力，构建大鼠牙齿移动模型。安乐死后采集组织样本，采用苏木精-伊红（hematoxylin and eosin, HE）染色法与抗酒石酸酸性磷酸酶（tartrate-resistant acid phosphatase, TRAP）染色法进行处理与染色，并对破骨细胞、霍夫陷窝（Howship’s lacunae）与血管进行计数。结果显示，与对照组相比，N组与D组的牙周膜重建进程显著加快。组织学变化的峰值在对照组为正畸装置安放后第5天，在N组与D组则为第3天。N3亚组与C3亚组、N3亚组与D3亚组之间的破骨细胞数量差异具有统计学意义（p<0.05）。超生理剂量的合成代谢雄激素类固醇（AAS）Deposteron®与Nebido®可改变破骨细胞、霍夫陷窝与血管的数量，加速牙周膜重建进程，从而加快大鼠正畸诱导牙齿移动的生物学效应。