Additional file 1: of Tumor regression mediated by oncogene withdrawal or erlotinib stimulates infiltration of inflammatory immune cells in EGFR mutant lung tumors

Figure S1. MRI images, histology and representative flow cytometry plots of normal or tumor-bearing lungs before and after erlotinib. (A) Coronal images of CCSPrtTA; TetO-EGFRL858R mouse lungs before (left panel) and after (right panel) treatment with erlotinib. (B) Hematoxylin and eosin (H&E) stain of lungs from control (normal) and tumor bearing CCSP-rtTA; TetO-EGFRL858R mice in the absence (−) and presence (+) of erlotinib for 2 weeks. Bar: 50 μm. Absolute number of (C) CD4 and (D) CD8 T cells normalized to weight of lungs of control (normal) and tumor bearing CCSP-rtTA; TetO-EGFRL858R mice in the absence (−) and presence (+) of erlotinib for 2 weeks. Representative FACS plot showing percentage of (E) FoxP3+ and FoxP3- CD4+ T cells (F) PD1+ FoxP3+ T cells. (G) Immunofluorescence (IF) stain of lung epithelial cells (green), CD3 T cells (red) and FoxP3 Tregs (Cyan). Nuclei were counterstained with Dapi (blue) (H) Quantification of FoxP3+ CD3 T cells in lung tumor bearing CCSP-rtTA; TetO-EGFRL858R mice in the absence (−) and presence (+) of erlotinib for 2 weeks stained by IF. Data are shown as mean ± SD and * is P

补充图S1 厄洛替尼处理前后正常或荷瘤肺部的磁共振成像（Magnetic Resonance Imaging, MRI）图像、组织学切片及代表性流式细胞术图谱。(A) 经厄洛替尼处理前（左图）及处理后（右图）的CCSP-rtTA; TetO-EGFRL858R小鼠肺部冠状位MRI图像。(B) 未给予（−）及给予（+）厄洛替尼处理2周后，对照组（正常）及荷瘤CCSP-rtTA; TetO-EGFRL858R小鼠肺部的苏木精-伊红（Hematoxylin and eosin, H&E）染色结果。标尺：50 μm。(C) 及(D) 分别为未给予（−）及给予（+）厄洛替尼处理2周后，对照组（正常）及荷瘤CCSP-rtTA; TetO-EGFRL858R小鼠肺部CD4⁺T细胞与CD8⁺T细胞的绝对计数（以肺部重量标准化后的数值）。(E) 代表性荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）散点图，展示叉头框蛋白P3（Forkhead box P3, FoxP3）⁺与FoxP3⁻ CD4⁺T细胞的占比；(F) 程序性死亡蛋白1（Programmed Cell Death Protein 1, PD1）⁺ FoxP3⁺ T细胞的占比。(G) 肺部上皮细胞（绿色）、CD3⁺T细胞（红色）及FoxP3⁺调节性T细胞（青色）的免疫荧光（Immunofluorescence, IF）染色结果。细胞核经4',6-二脒基-2-苯基吲哚（4',6-Diamidino-2-Phenylindole, DAPI）复染（蓝色）。(H) 免疫荧光染色后，对未给予（−）及给予（+）厄洛替尼处理2周的荷瘤CCSP-rtTA; TetO-EGFRL858R小鼠肺部FoxP3⁺CD3⁺T细胞的定量分析结果。数据以均值±标准差（Standard Deviation, SD）展示，*代表P