Sample extraction and SNP sequencing data for: Identification of sex-linked SNP markers in wild populations of monomorphic birds

Single-nucleotide polymorphism (SNP) analyses are a powerful tool for population genetics, pedigree reconstruction and phenotypic trait mapping. However, the untapped potential of SNP markers to discriminate the sex of individuals in species with reduced sexual dimorphism or of individuals during immature stages remains a largely unexplored avenue. Here, we develop a novel protocol for molecular sexing of birds based on the detection of unique Z- and W-linked SNP markers. Our method is based on the identification of two unique loci, one in each sexual chromosome. Individuals are considered males when they show no calls for the W-linked SNP and are heterozygotic or homozygotic for the Z-linked SNP, while females show both Z- and W-linked SNP calls. We validated the method in the Jackdaw (Corvus monedula). The reduced sexual dimorphism in this species makes it difficult to sex individuals in the wild. We assessed the reliability of the method using 36 individuals of known sex and found th..., ,

单核苷酸多态性（Single-nucleotide polymorphism, SNP）分析是开展群体遗传学研究、谱系重建以及表型性状定位的强有力工具。然而，SNP标记在性二态性较弱的物种中鉴别个体性别，或是在个体未成熟阶段判定其性别的未开发潜力，目前仍在很大程度上未被探索。本研究开发了一种基于特异性Z连锁与W连锁SNP标记检测的鸟类分子性别鉴定新方法。该方法通过鉴定两个分别位于不同性染色体上的特异性基因座实现：当个体未检出W连锁SNP位点，且Z连锁SNP位点呈杂合或纯合状态时，判定为雄性；而雌性个体则可同时检出Z连锁与W连锁SNP位点。本研究以寒鸦（Corvus monedula）为对象对该方法进行了验证，该物种种内性二态性较弱，野外难以直接鉴别个体性别。我们利用36例已知性别的个体对该方法的可靠性进行了评估，结果发现……