MYC and HSF1 Cooperate to Drive PLK1 inhibitor Sensitivity in High Grade Serous Ovarian Cancer

MYC and HSF1 genes are amplified in approximately 1/3 of ovarin cancer patients. However, their molecular interactions and cooperation to drive ovarian cancer has not been studied nor has their DNA binding patterns been compared in ovarian cancer cells. This project studies the interactions between MYC and HSF1 in ovarian cancer cells, including identifying their binding patterns across the genome for comparison. Ovarian cancer cells with MYC-HSF1 co-amplification (OVCAR8) underwent CUT&RUN for IgG, MYC, and HSF1 followed by sequencing. Overall design: OVCAR8 cells were subjected to CUT&RUN for IgG, MYC, and HSF1 followed by sequencing.