Continuous passive and active fluorescence measurements of chlorophyll-a (Chl), phycoerythrin (PE), chromophoric dissolved organic matter (CDOM), and variable fluorescence (Fv/Fm) from CCE process cruises in the California Current System, 2012 - 2019 (ongoing).

Active and passive fluorescence measurements are made using the ALFA5 system (Chekalyuk and Hafez, 2013) on water from the ship’s underway system during CCE process cruises. The instrument uses excitation at 405 and 510 nm to measures passively the fluorescence of chlorophyll-a (Chl), three different phycoerythrins (PE1, PE2 and PE3) and chromophoric dissolved organic matter (CDOM). Variable fluorescence (Fv/Fm) is measured actively using pump-during-probe (PDP) measurements of Chl a fluorescence induction. Fluorescence measurements are normalized to the water’s Raman fluorescence.

本研究在CCE过程航次期间，采用ALFA5系统（ALFA5 system，Chekalyuk与Hafez, 2013）对取自船舶走航取水系统的水样开展主动与被动荧光测量。该仪器采用405 nm与510 nm激发光，被动检测叶绿素a（chlorophyll-a，Chl）、三种不同藻红蛋白（phycoerythrins，PE1、PE2与PE3）以及有色溶解有机物（chromophoric dissolved organic matter，CDOM）的荧光信号。可变荧光（Fv/Fm）采用泵入式探头法（pump-during-probe，PDP）开展叶绿素a荧光诱导的主动测量。所有荧光测量结果均以水样的拉曼荧光信号进行归一化处理。