Intra- and interspecific toxicity data for nematodes exposed to metals

Twenty-four hour median lethal concentration (24hr-LC50) toxicity tests were performed with five species of nematodes (Caenorhabditis elegans, Caenorhabditis briggsae, Pristionchus pacificus, Oscheius tipulae, and Oscheius myriophila) in response to copper chloride and zinc chloride. In addition, lethality tests were also performed with seven strains of C. elegans (N2 >1 year in culture, N2 newly acquired, N2 ancestral, ED3053, JU258, JU1171, and MY1) exposed to copper chloride. Nominal chemical concentrations were validated and analyzed according to U.S. Environmental Protection Agency method 6010 using inductively coupled plasma–atomic emission spectroscopy (ICP-AES). Files with raw toxicity data, LC50 calculations, and chemical concentration validation are all reported here. The results indicate that P. pacificus is the most sensitive species to both metals. Conversely, C. elegans is the least sensitive species to copper, but the second most sensitive to zinc, indicating that species relationships do not necessarily predict species sensitivity. We also found that the four wild strains of C. elegans are more sensitive than standard laboratory N2 strains to copper, especially the N2 strain that was cultured in the lab for >1 year, suggesting that long-term lab strains may be less sensitive to copper than their wild counterparts.

本数据集针对氯化铜与氯化锌两种污染物，针对5种线虫完成了24小时半数致死浓度（24hr-LC50）毒性测试，受试物种包括秀丽隐杆线虫（Caenorhabditis elegans）、布氏隐杆线虫（Caenorhabditis briggsae）、太平洋小杆线虫（Pristionchus pacificus）、蒂普拉奥施杆线虫（Oscheius tipulae）以及多孢奥施杆线虫（Oscheius myriophila）。此外，本研究还针对7株秀丽隐杆线虫（实验室培养超1年的N2株、新获取的N2株、祖先N2株、ED3053、JU258、JU1171及MY1）开展了氯化铜暴露下的致死性测试。所有标称化学浓度均按照美国环境保护署（U.S. Environmental Protection Agency）方法6010，借助电感耦合等离子体原子发射光谱法（inductively coupled plasma–atomic emission spectroscopy，ICP-AES）完成验证与分析。本数据集公开了原始毒性数据、半数致死浓度计算结果以及化学浓度验证相关文件。研究结果显示，太平洋小杆线虫对两种金属均表现出最高的敏感性；与之相对，秀丽隐杆线虫对铜的敏感性最低，但对锌的敏感性位居第二，这表明物种间的演化亲缘关系未必能准确预测其对毒物的敏感性。此外，本研究还发现4株野生秀丽隐杆线虫对铜的敏感性高于标准实验室N2株，其中尤以实验室培养超1年的N2株最为明显，提示长期实验室传代的菌株对铜的敏感性可能低于其野生同源菌株。