Toehold-Mediated Nonenzymatic DNA Strand Displacement analysis dataset

This dataset comprises the results of analysis of toehold-mediated DNA strand displacement. The results demonstrate a new, simpler approach for the analysis of real-life DNA samples using a toehold-mediated DNA strand displacement reaction where a partially complementary duplex, with a ss-toehold sequence, was used as a target for analysis.<br><br>The approach was successfully implemented for human DNA genotyping for gender identification and it is envisaged that this model system could be easily adapted to other DNA genotyping molecular diagnostics.<br><br>Human genomic and mitochondrial DNA were coextracted from the author’s own blood samples. The analysis of this DNA consisted of two stages:<br>(i) PCR amplification was used to generate a PCR product with a contiguous ss-toehold domain; and<br>(ii) toehold-mediated DNA strand displacement reactions between the target toehold-PCR product and single-stranded chemically synthesized oligonucleotide molecular probes were performed using a quenched Föster resonance energy transfer (FRET) technique to monitor how the reaction proceeded.Copyright (c) 2013 Flinders University, South Australia<br><br>Date coverage: 2012-01-01 - 2012-06-01

本数据集收录了toehold介导的DNA链置换反应（toehold-mediated DNA strand displacement）的分析结果。研究结果表明，依托此类链置换反应，可构建一套全新且更简便的实用DNA样本分析方案：以带有ss-toehold序列的部分互补双链作为分析靶标。

该方法已成功应用于性别鉴定相关的人类DNA基因分型工作，且预计该模型系统可便捷适配其他DNA基因分型分子诊断场景。

本研究作者从自身血液样本中同步提取了人类基因组DNA与线粒体DNA。该样本的分析分为两个阶段：
(i) 通过聚合酶链式反应（PCR）扩增得到带有连续ss-toehold结构域的PCR产物；
(ii) 以靶标ss-toehold-PCR产物与化学合成的单链寡核苷酸分子探针为反应底物，采用淬灭型福斯特共振能量转移（Föster resonance energy transfer, FRET）技术监测反应进程。

版权所有©2013 南澳大利亚弗林德斯大学
数据覆盖时段：2012年1月1日 — 2012年6月1日