CIL:11959

This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. The spindle and its orientation are made visible by the use of polarizing optics, and in this sequence, rotation of the spindle causes a temporary reversal of its contrast from bright to dark. When the needle is withdrawn, the spindle returns to its original position, but the opposite pole is now attached to the cortex.

本数据集为4组成组延时摄影影片（CIL 11958-11962）之一，展示了减数分裂卵母细胞纺锤体（meiotic oocyte spindle）对玻璃微针（glass microneedle）实施的各类显微操作的响应过程。该微针从下方刺入，穿过卵皮质（egg cortex）抵达中期纺锤体（metaphase spindle）的内极。借助偏振光学（polarizing optics）可使纺锤体及其取向清晰显现，在本次拍摄序列中，纺锤体旋转会导致其对比度暂时由亮转为暗。当微针拔出后，纺锤体将回到初始位置，但此时其相对的一极已连接至卵皮质。