Data from a toxicity test using an Antarctic rotifer (Philodina sp.) exposed to copper in water

This data file contains results from a single toxicity test using an Antarctic rotifer (Philodina sp.) exposed to copper. The test was run at the Casey research station laboratory in east Antarctica during 2018/2019 summer. Test biota (rotifers; Philodina sp.) were isolated from soil and moss samples collected from Casey and maintained in a temperature controlled cabinet at 10°C with 12/12 hour light dark cycle for 1-2 weeks prior to testing. Test solution was Ultrapure water, spiked with copper stock solutions as necessary. The test was conducted in a 48 microwell plate and consisted 5 copper treatments and a control, replicated four times. Each treatment well contained 10-13 individual rotifersNominal test concentrations were as follows: Cu 0, 10, 130, 200, 400 and 600 µg/LThe test ran for 3 days, with rotifer activity and death assessed via light microscope at 48h and 96hAt the end of the test period samples were pooled by treatment and acidifed to 10% HNO3, then analysed for copper concentration via ICP-MS.

本数据集包含一项以南极轮虫（rotifer，Philodina sp.）为受试生物、暴露于铜的单一毒性试验结果。该试验于2018/2019年夏季在东南极凯西研究站实验室开展。受试生物（轮虫，Philodina sp.）采自凯西周边的土壤与苔藓样本，并于试验前在温度可控的培养箱中以10℃、12小时光照/12小时黑暗的光周期驯养1~2周。试验溶液采用超纯水，按需添加铜标准储备液进行配制。本试验采用48孔板开展，设置5个铜处理组与1个对照组，每组设置4个重复。每个处理孔接种10~13只轮虫。试验名义浓度设置如下：铜浓度分别为0、10、130、200、400及600 μg/L。试验周期为3天，分别于48小时和96小时通过光学显微镜评估轮虫的活动状态与存活情况。试验结束后，按处理组混合样本，用硝酸酸化至10%（体积分数）的硝酸体系，随后通过电感耦合等离子体质谱法（ICP-MS）测定铜浓度。