Deep sequencing analyzed the coverage of sgRNA in haploid stem cells.

We used the deep sequencing to analyze the sgRNA coverage of Dnd1 library in 4B2N1-DP1 and 4B2N1-DP2 cell lines. The amplicon obtained from PCR reaction from genome using high-fidelity DNA polymerase. By obtaining over 10 million reads of each sample from deep sequencing, we mapped the reads to the reference sequence and then calculated coverage and counts. The results showed that the hit number of different sgRNAs were variable Deep sequencing analyzed two different cell lines.