Drugs susceptibility profile (EC50 values and Fold-change) to the antileishmanials in all polyene resistant lines of Leishmania spp.

Mean EC50 values (μM) ± Standard Deviation (SD). Except for NMC tested in duplicates, values are from at least three biological replicates. One-way ANOVA was performed independently for each compound to determine differences of the mean between groups. Tukey’s test compared pairwise differences of resistant lines with respect the parental wild type. Statistical difference (P0.05; * P≤0.05; ** P≤0.01; *** P≤0.001; **** P≤0.0001.). ND: not determined. AmB (amphotericin B), NMC (natamycin), Nys (nystatin), keto (ketoconazole), MF (miltefosine), PAR (paromomycin), PAT (antimony potassium tartrate), PENT (pentamidine), MB (methylene blue). See Material and Methods for full details. Fold change in EC50 is relative to the parental to the respective parental wild type cultured in parallel without drug. Values higher and lower than 1, indicate a decrease and increase in susceptibility, respectively. (XLSX)