Palmitoylation negatively regulates AEG-1 function

In vitro studies revealed that AEG-1 is palmitoylated at cysteine at a.a. 75 position. In order to check how palmitoylation regulates AEG-1 function in vivo we generated a knockin moue using CRISPR/Cas9 taking in which cysteine 75 was mutated to serine (AEG-1-C75S). Hepatocytes were isolated from AEG-1-WT and AEG-1C75S littermates, total RNA was isolated and RNA-sequencing was performed. AEG-1-C75S heterozygotes were mated to generate AEG-1-WT and AEG-1-C75S homozygote mice. Hepatocytes were isolated from adult (6-8 wks old mice) and total RNA was extracted 48 h after isolation using QIAGEN RNA extraction kit. Total RNA was subjected to RNA-seq using Illumina platform.