Comparison of anatomical structures highlighted by Mn²⁺ over time in NET KO and wildtype littermate mice; and at one day post Mn²⁺ injection in DAT and SERT KO mice and their respective wildtype controls.

Manganese accumulation over time in NET KO mice compared to wildtype littermates follows a similar general time-course as seen in the SERT and DAT KO strains. Wildtype littermates exhibit more widespread Mn2+ induced hyperintensities than the NET KO mice at all times post-injection in PFC. At the 24 hour time point, the pattern of manganese accumulation is significantly restricted in the NET KO compared to wildtype mice. I subscript: ipsilateral, C subscript: contralateral, WT: wildtype only, KO: knockout only, B: both wildtype & knockout. Differences are considered significant at p<0.001 (FDR corrected) level.