Deep Sea Sponge and Seawater Targeted Locus (Loci)

The microbiota of four individual deep water sponges, Lissodendoryx diversichela, Poecillastra compressa, Inflatella pellicula, and Stelletta normani, together with surrounding seawater were analysed by pyrosequencing of a region of the 16S rRNA gene common to Bacteria and Archaea. The microbial communities of L. diversichela, P. compressa and I. pellicula were typical of low microbial abundance (LMA) sponges while S. normani was found to have a community more typical of high microbial abundance (HMA) sponges. Analysis of the deep sea sponge microbiota revealed that the three LMA-like sponges shared a set of abundant OTUs that were distinct from those associated with sponges from shallow waters.

本研究针对4株深海海绵个体（分别为Lissodendoryx diversichela、Poecillastra compressa、Inflatella pellicula及Stelletta normani）及其周边海水的微生物组，通过对细菌与古菌共有的16S rRNA基因区域进行焦磷酸测序（pyrosequencing）开展分析。其中，L. diversichela、P. compressa与I. pellicula的微生物群落具有低微生物丰度（low microbial abundance, LMA）海绵的典型特征，而S. normani的微生物群落则更符合高微生物丰度（high microbial abundance, HMA）海绵的群落特征。对深海海绵微生物组的分析结果显示，这3株低微生物丰度型海绵共享一组丰富的操作分类单元（Operational Taxonomic Unit, OTU），且该类群与浅海海绵关联的微生物类群存在显著差异。